EXPLOR™ Platform Technology
EXPLOR™ is the abbreviation for ‘Exosome engineering for Protein loading via Optically reversible Protein-Protein interaction’. (Publication: Nature Communications, 2016 volume7, Article number: 12277)EXPLOR™ is a protein cargo loading platform technology into exosome, utilizing CRY2 and CIBN as Protein-Protein Interaction module.
Originally, photoreceptor CRY2(Cryptochrome2) and CIBN(a truncated version of Cryptochrome Interacting Basic-Helix-Loop-Helix1) proteins were identified as light-sensing proteins in a flowering plant, Arabidopsis thaliana. In response to blue light illumination, these proteins show reversible binding to each other, which can be utilized to load cargo protein into exosome more efficiently.
Exosome producing cells are genetically engineered to express two different sets of protein complex: CRY2-conjugated cargo protein and CIBN-conjugated exosome membrane-associated protein (e.g. CD9). During exosome biogenesis, exosome producing cells are cultured under blue light to make the cargo proteins dragged into the exosomes via CRY2-CIBN interaction. After the cargo-loaded exosomes are produced from the cells, the blue light is removed, and the exosomes are isolated/purified from cell culture media and further concentrated to be used as therapeutics.
We believe that our innovative EXPLOR™ technology allows us to generate exosome-encapsulated API protein with high loading yield which stably delivers API protein into the target cells in various diseases with significant medical unmet needs.